heterozygous sert cre mice  (Jackson Laboratory)

Journal: bioRxiv

Article Title: Alterations of adult prefrontal circuits induced by early postnatal fluoxetine treatment mediated by 5-HT7 receptors

doi: 10.1101/2023.11.30.569458

Figure Lengend Snippet: a: Confocal fluorescence micrograph illustrating a section of the PFC of a SERT-Cre::tdTomato adult mouse. Note the dense labeling of deep-layer tdTomato-positive neurons. Scale bar: 500 µm b: Close-up of the micrograph of A showing deep-layer, fluorescent SERT+ cells and shadows of SERT- cells (asterisks). Scale bar: 10 µm. c: Scheme of the experimental procedure. PNFLX protocol was followed by patch-clamp recordings in acute brain slices obtained from adult mice. d: Representative current-clamp traces showing the response to 0.8 s-long current injections from SERT+ and SERT- PNs. Red and orange traces: SERT+ PNs in CTR and PNFLX, respectively. Black and gray traces: SERT- PNs in CTR and PNFLX, respectively. current injections: from -100 to +375 pA in all cases. e: Firing rate vs. current injections in all cases. Same colors as in D. *P < 0.05; **P < 0.01; ***P < 0.001. f: Scheme of the experimental procedure. Same as in C, except that during PNFLX protocol mice received concomitant intraperitoneal injections of saline solution or saline solution containing the 5-HT7 antagonist (SB269970) twice a day. g-h: Same as in d-e but in the presence of SB269970 (SB).

Article Snippet: In order to record SERT+ pyramidal neurons we crossed B6.129(Cg)- Slc6a4 tm1(cre)Xz /J (Sert-Cre) mice with B6.Cg-Gt(ROSA)26Sor/J mice (td-Tomato) (The Jackson Laboratory, Bar Harbor).

Techniques: Fluorescence, Labeling, Patch Clamp, Saline

Journal: bioRxiv

Article Title: Alterations of adult prefrontal circuits induced by early postnatal fluoxetine treatment mediated by 5-HT7 receptors

doi: 10.1101/2023.11.30.569458

Figure Lengend Snippet: a-b: Fluorescent micrographs showing a SERT+ (left) and a SERT- (right) PN filled with biocytin and counterstained with green, fluorescent streptavidin. Neurons were filled in a SERT-Cre::TdTomato mouse. Red fluorescence indicates SERT+ PNs. Scale bar 50 µm. c: Representative somatodendritic reconstructions of biocytin-filled SERT+ and SERT- PNs in CTR and PNFLX. Scale bar 100 µm. d: Number of intersections plotted as a function of the distance from soma for basal (lef) and apical (right) dendrites for the two cell types (SERT+ and SERT-), for both treatments (CTR and PNFLX). The yellow box corresponds to the data points that are significantly different between SERT+ and SERT- PNs (* : P<0.005).

Article Snippet: In order to record SERT+ pyramidal neurons we crossed B6.129(Cg)- Slc6a4 tm1(cre)Xz /J (Sert-Cre) mice with B6.Cg-Gt(ROSA)26Sor/J mice (td-Tomato) (The Jackson Laboratory, Bar Harbor).

Techniques: Fluorescence

Journal: bioRxiv

Article Title: Alterations of adult prefrontal circuits induced by early postnatal fluoxetine treatment mediated by 5-HT7 receptors

doi: 10.1101/2023.11.30.569458

Figure Lengend Snippet: a: Representative voltage-clamp traces of glutamatergic sEPSCs recorded from PFC PNs of adult CTR and PNFLX-treated mice. b: Population data of sEPSC frequency (left) and amplitude (right) in SERT+ and SERT- PNs in CTR and PNFLX-treated mice. Green and black lines indicate median and mean values. resepctively. c-d: Same as in a-b but for GABAergic sIPSCS, isolated using a high-chloride intracellular solution and in the continuous presence of ionotropic glutamate receptor antagonists in the extracellular superfusate

Article Snippet: In order to record SERT+ pyramidal neurons we crossed B6.129(Cg)- Slc6a4 tm1(cre)Xz /J (Sert-Cre) mice with B6.Cg-Gt(ROSA)26Sor/J mice (td-Tomato) (The Jackson Laboratory, Bar Harbor).

Techniques: Isolation